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A
Quantitative Assessment of Bystander Mutagenesis in the Mouse
Mammary Gland In vivo
Amy Kronenberg
a_kronenberg@lbl.gov
Lawrence Berkeley National Laboratory
Website:
http://www.lbl.gov/lifesciences/labs/kronenberg_lab.html
Why
this Project?
To quantify
gene conversion in bystander cells from the mouse mammary
gland in vivo following stromal irradiation of the fat pads.
Project
Goals:
- To define
the baseline frequencies of gene conversion in bystander cells.
- To assess
the role of these genetic alterations in mammary cancer.
Experimental Approach:
A DR-Green
Fluorescent Protein (GFP) mouse model will be used in these
studies. The bystander effects will be produced by irradiation
of the fat pad prior to transplantation with non-exposed DR-GFP
mammary epithelial cells. The detection of EGFP+ mammary epithelial
cells represents gene conversion and provides an indicator
of the repair of replication errors via homologous recombinational
repair. Gene changes in bystander cells will be counted using
flow cytometry as well as using an innovative quantitative
microscopy.
Expected
Outcomes:
These
studies will aid in development of models for the type of
gene conversion asoociated with the induction of breast cancer
in women exposed to low doses of radiation. Such studies will
be important in estimating risk for radiation-induced breast
cancer.
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